Al Irvine
New Graph Environment Ltd.

250-777-1518
Date Original: 2025-07-15
Date Revised: 2025-07-28

Ministry of Environment


Re: Scientific Fish Collection Permit Application

This permit application can also be viewed online at this link. A summary of sites proposed for assessment, including historic fish presence records from FISS, is provided in Tables 2 to 3. Fish species known to occur within each watershed are summarized in Table 4. An overview map showing potential sample locations is presented in Figure 1. A KML file (google earth) and GPX file (for garmin gps devices) of all sites is attached to the application with latest versions downloadable here or here. The KML includes detailed site-specific information accessible by clicking on each location in google earth, with brief summaries of background reports where available.


Brief description of project/activities

This work is a multi-year collaboration of many groups and an initiative of the Society for Ecosystem Restoration Northern BC. Funding for the project is through the Fish and Wildlife Compensation Program, SERNbc and the Ministry of Transportation and Infrastructure. Fieldwork is being led by Al Irvine, R.P.Bio., of New Graph Environment Ltd., in collaboration with field and office teams from McLeod Lake Indian Band. Previous reports are linked below:




Rationale for sampling

Rationale for sampling is to inform fish presence/absence, species composition/density, abundance estimates, movement, growth, and survival as part of habitat confirmations and effectiveness monitoring related to fish passage restoration at barrier culverts. Habitat confirmation methodology information can be referenced in the above reports which builds on the Fish Passage Technical Working Group Phase 2 protocol. Presence/absence of fish, species composition/abundance, distribution limits and fish movement can be useful for prioritizing which crossings are a best fit for fish passage restoration and inform baseline as well as follow up effectiveness monitoring.


Methodologies

Sampling methodologies will be dependent on the site, fish species suspected, type of habitat encountered, risks to aquatic organisms potentially present (Table 1) and ongoing communications. Sampling methods may include minnowtrapping, electrofishing, and dip netting upstream and downstream of current and past barrier culvert locations.


Sampling is proposed at streams included in Tables 2 - 3 where we will be performing habitat confirmation assessments and follow up site visits related to past habitat confirmations/fish passage remediations.


PIT Tagging

As part of this permit application we are proposing PIT tagging. When time allows and tagging is expected to improve knowledge of a system, our study plan is to electrofish small sites both upstream and downstream of priority culvert “barrier” sites and implant Biomark APT12 PIT tags in the abdominal cavity of select fish over 60mm in fork length. To anesthetize fish prior to PIT tagging, we use a clove oil solution at 0.1mL/L (1:10,000), which provides effective sedation with minimal residual effects (Fernandes et al. 2017). The solution is prepared by dissolving clove oil in ethyl alcohol at a 1:9 ratio before mixing into water (Fernandes et al. 2017). Site location (UTM), fish length and weight will also be collected. In addition to providing information on abundance upstream and downstream of potential culvert restoration sites, the study will also provide information for monitoring programs to document fish movement, growth and survival at sites over multi-year time frames. Main objectives are to:

  1. Determine if fish are moving into restored areas
  2. Determine if before any remediation is conducted - fish are moving through sites where stream crossing structures (culverts) likely cause connectivity issues
  3. Evaluate if productivity of the systems are increasing following bridge installation and/or if fish are moving upstream/downstream of where replaced/removed structures are located


Dependent on how relevant tracking information would be to inform restoration actions, we may wish to tag select fish over 60mm in each site sampled. We would like to apply for a permit allowing a maximum of 600 fish tagged with a maximum of 150 fish/stream. Although we are requesting a maximum of 150 fish/stream, we have listed 150 fish of each species per stream because we will not know the species composition of the sites until the sampling occurs. In general, only salmonid and burbot species will be tagged with likely species present being rainbow trout, bull trout, and burbot. Based on past assessments in the same and similiar systems in the region, the number of fish tagged per stream are very likely to be much less than 150, however we are requesting the maximum number of fish to be tagged to facilitate permit application procedures and allow for flexibility in the field based on actual sampling results.


Risks associated with project/activities and associated mitigation

Table 1: Risks and mitigation
Impact Mitigation
High Voltage Injuries Use the minimum effective voltage. Avoid contacting fish with the anode. Avoid electrofishing directly adjacent to metal culverts.
Disruption of Spawning Avoid electrofishing during highest risk periods in likely spawning habitat.
Physical Stress on Fish Quick/gentle handling and release of captured fish. Use of clove oil to anesthetize fish.
Injury from PIT Tagging Surgeries Shallow insertion of tags and use of fresh sterile syringes every approximately 10 surgeries.
Mortality in traps due to predation and starvation Ensure all traps set are retrieved within 24 hours.


Please note that the sampling will be completed before October 31 (end of August till early October) however the end-date of the sampling period is listed as Dec 31 on the application to allow time outside of the busy field season for the data to be processed, QA’d and organized so that required reporting can be as informative as possible when submitted. An example of how we have been presenting results and methodologies from past assessments can be referenced in reports above.


Please do not hesitate to contact me if you need more information or have any questions or concerns.

signature Al Irvine, R.P.Bio

Location of potential sample sites.

Figure 1: Location of potential sample sites.

Table 2: Potential sampling locations.
Site ID Stream Name Watershed Code UTM Zone UTM Easting UTM Northing Watershed Group Code
125000 Tributary To Parsnip River 236-738000-00000-00000-0000-0000-000-000-000-000-000-000 10 577541 6038215 PARS
125179 Unnamed Tributary To Missinka River 236-614900-20900-03300-0000-0000-000-000-000-000-000-000 10 570307 6052836 PARS
125180 Tributary To Missinka River 236-614900-20900-00000-0000-0000-000-000-000-000-000-000 10 569665 6053046 PARS
125231 Tributary To Table River 236-450800-15400-00000-0000-0000-000-000-000-000-000-000 10 549962 6065137 PARS
125261 Fern Creek 236-358400-00000-00000-0000-0000-000-000-000-000-000-000 10 534601 6067771 PARS
125749 Unnamed Tributary To Airline Creek 237-625800-53700-00000-0000-0000-000-000-000-000-000-000 10 374238 6102796 NATR
125755 Glaucers Creek 237-671800-00000-00000-0000-0000-000-000-000-000-000-000 10 359563 6116606 NATR
15200034 237-792500-61000-00000-0000-0000-000-000-000-000-000-000 10 357257 6160917 NATR
15200384 237-528900-57600-16500-2290-0000-000-000-000-000-000-000 10 403312 6145393 NATR
15200412 Gillis Creek 237-528900-57600-00000-0000-0000-000-000-000-000-000-000 10 400259 6152248 NATR
15200939 Nation River 237-000000-00000-00000-0000-0000-000-000-000-000-000-000 10 325553 6152065 NATR
15200985 237-077300-00000-00000-0000-0000-000-000-000-000-000-000 10 458491 6137652 NATR
15201007 237-062300-00000-00000-0000-0000-000-000-000-000-000-000 10 459777 6140085 NATR
15201341 237-449000-00000-00000-0000-0000-000-000-000-000-000-000 10 408422 6121011 NATR
15201343 237-393200-00000-00000-0000-0000-000-000-000-000-000-000 10 417680 6121619 NATR
15201728 237-897400-00000-00000-0000-0000-000-000-000-000-000-000 10 330395 6149308 NATR
15201740 Fish Creek 237-673600-00000-00000-0000-0000-000-000-000-000-000-000 10 355086 6123605 NATR
15201834 237-688000-00000-00000-0000-0000-000-000-000-000-000-000 10 362065 6124875 NATR
15202084 237-124500-19300-00000-0000-0000-000-000-000-000-000-000 10 460163 6130113 NATR
15202950 237-713900-19200-08800-0000-0000-000-000-000-000-000-000 10 361739 6134520 NATR
15203146 237-877600-00000-00000-0000-0000-000-000-000-000-000-000 10 334061 6150367 NATR
16400428 230-906900-15800-00000-0000-0000-000-000-000-000-000-000 10 488458 6112359 PARA
16400714 230-910500-00000-00000-0000-0000-000-000-000-000-000-000 10 469038 6148922 PARA
16400738 230-905500-00000-00000-0000-0000-000-000-000-000-000-000 10 491471 6131967 PARA
16401519 230-907800-00000-00000-0000-0000-000-000-000-000-000-000 10 486512 6124976 PARA
16401530 230-907400-00000-00000-0000-0000-000-000-000-000-000-000 10 486724 6116602 PARA
16401533 Dastaiga Creek 230-909100-00000-00000-0000-0000-000-000-000-000-000-000 10 479617 6141422 PARA
16401545 Blackwater Creek 230-913400-00000-00000-0000-0000-000-000-000-000-000-000 10 456256 6156862 PARA
16401547 230-910000-00000-00000-0000-0000-000-000-000-000-000-000 10 476480 6147560 PARA
16401699 Gagnon Creek 230-905800-00000-00000-0000-0000-000-000-000-000-000-000 10 491234 6125187 PARA
16401990 230-908900-00000-00000-0000-0000-000-000-000-000-000-000 10 480206 6138995 PARA
198668 Tributary To Mcleod Lake 230-906800-71800-00000-0000-0000-000-000-000-000-000-000 10 501971 6087814 CARP
24718358 Buth Creek 230-906600-00000-00000-0000-0000-000-000-000-000-000-000 10 499574 6115412 PARA
62623 Suschona Creek 237-372000-61800-00000-0000-0000-000-000-000-000-000-000 10 422422 6108185 NATR
6559 230-906900-02100-00000-0000-0000-000-000-000-000-000-000 10 489968 6112333 PARA
6564 230-906900-19500-00000-0000-0000-000-000-000-000-000-000 10 486579 6112299 PARA
Table 3: Potential sample site details
Site ID Stream Name Species Upstream # Fish Tags
125000 Tributary To Parsnip River 150
125179 Unnamed Tributary To Missinka River BT;RB 150
125180 Tributary To Missinka River RB 150
125231 Tributary To Table River RB 150
125261 Fern Creek BB;C;CBC;DC;DV;LSU;RB;RSC;SU 150
125749 Unnamed Tributary To Airline Creek 150
125755 Glaucers Creek BB;CC;CSU;LSU;NSC;RB;RB/CT;RSC;SP;SU 150
15200034 150
15200384 150
15200412 Gillis Creek 150
15200939 Nation River RB 150
15200985 150
15201007 RB 150
15201341 150
15201343 CCG;LKC;RB;SP 150
15201728 150
15201740 Fish Creek BT;RB 150
15201834 150
15202084 RB 150
15202950 150
15203146 150
16400428 PCC;RB 150
16400714 150
16400738 150
16401519 LKC;RB 150
16401530 RB 150
16401533 Dastaiga Creek SP 150
16401545 Blackwater Creek BB;BT;CC;CSU;DV;GR;LKC;LSU;MW;RB 150
16401547 RB;SP 150
16401699 Gagnon Creek BB;BMC;BT;C;CAS;CC;CCG;CSU;LKC;LSU;LW;NSC;PCC;RB;RSC;SP;SU;WSU 150
16401990 RB 150
198668 Tributary To Mcleod Lake 150
24718358 Buth Creek 150
62623 Suschona Creek CSU;LKC;LSU;RB 150
6559 150
6564 150


Table 4: Fish species recorded in the Fisheries Information Summary System within the freshwater atlas watershed group areas where the potential sample sites are located.
Scientific Name Species Name BC List COSEWIC Carp Lake Crooked Nation Parsnip Arm Parsnip
Catostomus catostomus Longnose Sucker Yellow Yes Yes Yes Yes Yes
Catostomus columbianus Bridgelip Sucker Yellow Yes
Catostomus commersonii White Sucker Yellow Yes Yes Yes Yes Yes
Catostomus macrocheilus Largescale Sucker Yellow Yes Yes Yes Yes Yes
Chrosomus eos Northern Redbelly Dace Yellow
Chrosomus neogaeus Finescale Dace Yellow
Coregonus clupeaformis Lake Whitefish Yellow Yes Yes Yes Yes Yes
Cottus aleuticus Coastrange Sculpin (formerly Aleutian Sculpin) Yellow Yes
Cottus asper Prickly Sculpin Yellow Yes Yes Yes Yes Yes
Cottus cognatus Slimy Sculpin Yellow Yes Yes Yes Yes
Cottus hubbsi Mottled Sculpin Blue SC (Nov 2010) Yes Yes
Cottus rhotheus Torrent Sculpin Yellow
Cottus ricei Spoonhead Sculpin Yellow NAR (May 1989) Yes
Couesius plumbeus Lake Chub Yellow DD Yes Yes Yes Yes Yes
Culaea inconstans Brook Stickleback Yellow
Esox lucius Northern Pike Yellow Yes
Hiodon alosoides Goldeye Blue
Hybognathus hankinsoni Brassy Minnow No Status Yes Yes Yes Yes
Lota lota Burbot Yellow Yes Yes Yes Yes Yes
Mylocheilus caurinus Peamouth Chub Yellow Yes Yes Yes Yes Yes
Notropis atherinoides Emerald Shiner Unknown
Notropis hudsonius Spottail Shiner Red
Oncorhynchus clarkii Cutthroat Trout No Status
Oncorhynchus clarkii lewisi Westslope (Yellowstone) Cutthroat Trout Blue SC (Nov 2016)
Oncorhynchus mykiss Rainbow Trout Yellow Yes Yes Yes Yes Yes
Oncorhynchus nerka Kokanee Yellow Yes Yes Yes
Osmerus dentex Rainbow Smelt Unknown Yes Yes
Perca flavescens Yellow Perch Unknown
Percopsis omiscomaycus Trout-perch Yellow
Platygobio gracilis Flathead Chub Yellow
Prosopium coulterii Pygmy Whitefish Yellow NAR (Nov 2016) Yes Yes Yes
Prosopium cylindraceum Round Whitefish Yellow Yes
Prosopium williamsoni Mountain Whitefish Yellow Yes Yes Yes Yes Yes
Ptychocheilus oregonensis Northern Pikeminnow Yellow Yes Yes Yes Yes Yes
Rhinichthys cataractae Longnose Dace Yellow Yes Yes Yes Yes
Rhinichthys falcatus Leopard Dace Yellow NAR (May 1990)
Richardsonius balteatus Redside Shiner Yellow Yes Yes Yes Yes Yes
Salvelinus confluentus Bull Trout Blue SC (Nov 2012) Yes Yes Yes Yes Yes
Salvelinus fontinalis Brook Trout Exotic Yes Yes Yes
Salvelinus malma Dolly Varden Yellow Yes Yes Yes Yes Yes
Salvelinus namaycush Lake Trout Yellow Yes Yes Yes Yes Yes
Sander vitreus Walleye Yellow
Thymallus arcticus Arctic Grayling Yellow Yes Yes Yes
Chub (General) Yes Yes Yes Yes
Dace (General) Yes Yes
Lamprey (General) Yes
Minnow (General) Yes Yes Yes Yes
Northern Pearl Dace
Salmon (General) Yes
Sculpin (General) Yes Yes Yes Yes Yes
Smelt (General) Yes
Squanga Yes
Sucker (General) Yes Yes Yes Yes Yes
Whitefish (General) Yes Yes Yes Yes Yes


References

Fernandes, I. M., Y. F. Bastos, D. S. Barreto, L. S. Lourenço, and J. M. Penha. 2017. “The Efficacy of Clove Oil as an Anaesthetic and in Euthanasia Procedure for Small-Sized Tropical Fishes.” Brazilian Journal of Biology = Revista Brasleira De Biologia 77 (3): 444–50. https://doi.org/10.1590/1519-6984.15015.